Raman-spectroscopy-based noninvasive microanalysis of native lignin structure.
Identifieur interne : 002931 ( Main/Exploration ); précédent : 002930; suivant : 002932Raman-spectroscopy-based noninvasive microanalysis of native lignin structure.
Auteurs : Pradeep N. Perera [États-Unis] ; Martin Schmidt ; Vincent L. Chiang ; P James Schuck ; Paul D. AdamsSource :
- Analytical and bioanalytical chemistry [ 1618-2650 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Lignine.
- composition chimique : Arabidopsis, Lignine.
- méthodes : Analyse spectrale Raman.
- Structure moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Lignin.
- chemistry : Arabidopsis, Lignin.
- methods : Spectrum Analysis, Raman.
- Molecular Structure.
Abstract
A new robust, noninvasive, Raman microspectroscopic method is introduced to analyze the structure of native lignin. Lignin spectra of poplar, Arabidopsis, and Miscanthus were recovered and structural differences were unambiguously detected. Compositional analysis of 4-coumarate-CoA ligase suppressed transgenic poplar showed that the syringyl-to-guaiacyl ratio decreased by 35% upon the mutation. A cell-specific compositional analysis of basal stems of Arabidopsis showed similar distributions of S and G monolignols in xylary fiber cells and interfascicular cells.
DOI: 10.1007/s00216-011-5518-x
PubMed: 22071606
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<front><div type="abstract" xml:lang="en">A new robust, noninvasive, Raman microspectroscopic method is introduced to analyze the structure of native lignin. Lignin spectra of poplar, Arabidopsis, and Miscanthus were recovered and structural differences were unambiguously detected. Compositional analysis of 4-coumarate-CoA ligase suppressed transgenic poplar showed that the syringyl-to-guaiacyl ratio decreased by 35% upon the mutation. A cell-specific compositional analysis of basal stems of Arabidopsis showed similar distributions of S and G monolignols in xylary fiber cells and interfascicular cells.</div>
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